牦牛RGS1基因克隆及其在发情周期卵巢中的表达

doi:10.11843/j.issn.0366-6964.2017.02.008

Abstract

Abstract:

The aim of this study was to clarify the expression characteristics of yak RGS1 gene and its expression pattern in ovaries and other tissues on the estrous cycle. The primer was designed based on the GenBank published Bos taurus RGS1 gene sequences, and the expression pattern of RGS1 was detected by RT-PCR in yak various tissues which include muscle, spleen, heart, kidney, stomach, small intestine, cerebellum, liver, lung and the ovarian during in different periods. The structure and function of RGS1 were analyzed by relate bioinformatics software. The mRNA expression level of RGS1 in different periods of yak estrus cycle were detected by qRT-PCR. The results showed that the yak RGS1 gene contained a 718 bp cDNA fragment, and had high homology with other mammals in nucleotide sequence through sequence alignment and phylogenetic tree, indicating that RGS1 is relatively conservative in the evolutionary process. The length of the yak RGS1 gene was 591 bp in CDS, encoding 196 amino acids, and the molecular weight was of 22.48 ku. The protein was predicted to be hydrophilic, non-secretory, lacking cross-membrane and signal peptide. Its secondary structure and tertiary structure was mainly composed of random α-helix and coil. RGS1 gene was expressed in various tissues of yak, and high abundance in small intestine, cerebellum, heart and stomach. qRT-PCR results showed that the mRNA expression level of RGS1 was significantly higher in luteal phase ovarian of yak than that in follicular phase and red stage（P<0.01). Meanwhile, the mRNA expression level of RGS1 was higher in follicular phase than that in red stage ovarian, but no significant difference (P>0.05). The RGS1 was significant differences expression among the 3 periods of yak ovarian in estrus cycle, which indicated that RGS1 involved in the estrous cycle of ovarian reproductive endocrine activities regulation.

CLC Number:

S829.9

HU Jia-jia, XIONG Xian-rong, SHI Xian, CAI Wen-yi, LAN Dao-liang, LI Jian. Cloning and Expression of RGS1 Gene in Yak Ovary during the Estrous Cycle[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(2): 252-259.

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References

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Cloning and Expression of RGS1 Gene in Yak Ovary during the Estrous Cycle

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